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# Measuring enzyme-controlled reactions

Enzymes are biological catalysts that speed up chemical reactions without causing lasting changes. They do this by lowering the activation energy needed for the chemical reaction to take place. Enzymes function both intracellularly and extracellularly in living organisms. Let's dive into enzyme-controlled reactions.

Catalysis - the process by which a catalyst (e.g., enzymes) increases the reaction rate.

Activation energy - the minimum energy needed to initiate a specific reaction.

There are two main types of enzyme-controlled reactions:

• Anabolic (making large new molecules)

• Catabolic (breaking a larger molecule into smaller molecules)

The activation energy is required with an enzyme and without an enzyme

The International System for units (SI) is in katal for enzyme activity. 1 katal = 1 mol s-1. However, this is way too high to measure the activity, so usually, 1 μmol min-1 (16.67 (2 d.p.) katal) is used instead.

When a unit is rounded, it is usually to significant figures (s. f.) or decimal places (d. p.).

For example, you have a number - 0.0055834. What will it be to 2 decimal places, and what will it be to 2 significant figures?

• 2 d. p. = 0.01 (places after the decimal).
• 2 s. f. = 0.0056 (the first number to appear other than 0).

Always round up if the number is more than 5; for example, 0.05 will become 0.1 (if 1 d. p. needed). Always round down if lower than 5; for example, 0.14 will be 0.1 (1 d. p.).

An enzyme unit is the amount of enzyme required to catalyse the transformation of 1 μmol of substrate per minute under specified pH and temperature conditions. The number of units per milligrams of protein catalysed represents an enzyme's specific activity.

The following methods can be used to track the progress of enzyme-catalysed reactions:

1. Calculating the rate at which a product is formed - the rate at which, using the substrate, a new product will be formed.

2. Calculating the rate at which a substrate vanishes - the rate at which a specific amount of substrate will be used up.

## Factors affecting enzyme-controlled reaction rates

Different factors can influence the rate of reaction, and these include:

• Temperature: with increasing temperature, the enzyme activity increases due to increased collisions between the molecules. However, too high of a temperature can cause an enzyme to denature.

• pH: different enzymes have different optimum pH levels that they can work best in. Changes in pH outside the optimum range can denature an enzyme.

• The concentration of the substrate: more substrate will equal more collisions with the enzyme; however, the rate does not continue to increase exponentially unless there is an unlimited number of enzymes. When all enzyme active sites are occupied, no more substrates can bind.

• The concentration of the enzyme: increasing the concentration of the enzyme will increase the rate of reaction up to a certain point unless there is an unlimited supply of substrate. When all substrate has been reacted with, the enzyme has nothing else to bind to.

• Presence of inhibitors: inhibitors can either inhibit the enzyme's active site (substrate cannot bind) or bind to the allosteric site (changes the active site).

• Enzyme activators: specific molecules that bind to enzymes to increase their activity.

Effects of substrate concentration

Denaturation: the process by which a protein loses its shape (changes in 3D/tertiary structure) and will no longer bind substrate effectively. This is because the active site has been altered.

Enzyme active site: a specific binding site for the substrate.

Allosteric site: a region of the enzyme where a molecule can bind and alter the active site.

You will find a more detailed explanation for the factors affecting enzyme activity in our Factors Affecting Enzyme Activity article.

## Investigating enzyme-controlled reactions - required practicals

Let's have a look at some examples of practicals you may encounter.

### The effect of temperature on the rate of reaction

In this practical, the rate of reaction of an enzyme-controlled reaction will be investigated using a powdered milk suspension. The independent variable on this occasion will be temperature.

Independent variable: a variable that is not affected by other variables.

Dependent variable: a variable that you are measuring and is influenced by the independent variable.

#### Material list

For this practical, you will need:

• Powdered milk suspension

• Trypsin solution (0.5 %)

• Distilled water

• Hydrochloric acid (HCl) (0.1 M)

You will use a variety of conical flasks, tubes and other equipment as needed. Your teacher will provide these.

Trypsin is an enzyme that aids with digestion. For this practical, trypsin will be the catalyst.

#### Method

First, we need to create our control and test solutions.

Making up control samples

Make two control samples using the steps below. These control samples will be used to compare to your test samples.

• Add 5 cm3 of milk suspension to two tubes.
• Add 5 cm3 of distilled water to one tube to represent no enzyme activity
• Add 5 cm3 of HCl to the other tube to hydrolyse (break down of water) the sample. This represents the presence of enzyme activity.

Making up test samples and measuring the rate of reaction

The goal is to measure the reaction rate; we will do this by timing how long it will take for the enzyme to affect the reaction at different temperatures. When all protein in the milk has reacted, the solution will become colourless.

So why does the milk go colourless? Milk contains casein which is a slow-digesting dairy protein. When casein is broken down by trypsin, it causes the milk to lose its colour.

As the temperature increases, molecules will move faster due to increased kinetic energy, which will allow more collisions between enzymes and substrates. However, if the temperature is too high, it will cause the enzyme to denature.

1. Add 5 cm3 of milk into three test tubes and place all into a water bath at 10ºc for 5 minutes.
2. Add 5 cm3 of trypsin to each and start the timer.
3. Time how long it takes for the solution to become colourless.
4. Repeat steps 1 to 3 at different temperatures, such as 20ºc, 30ºc and so on.

#### Calculating the rate of reaction

To help with data-keeping, we suggest you use the following table arrangement:

 Temperature (ºc) Time taken for casein to break down (s) Mean time (s) Rate (s-1) 10 Repeat 1 Repeat 2 Repeat 3 20

You will have three repeats for each temperature (you can have more if you prepare more test tubes). By adding and dividing the time by the number of tubes, you will calculate the mean time taken for the sample to hydrolyse.

Now that you have timed all the reactions, you can calculate the rate of reaction using the following equation:

Rate of reaction = 1 / mean time

For example, at 10ºc, the milk hydrolysed in 50s, 55s and 60s. This means, the mean time is (50 + 55 + 60) / 3 = 55s.

Let's put this into the rate of reaction equation:

1 / 55 = 0.02 (2 d. p.) s-1.

When you calculate rates of reactions for each temperature, you can plot them on the graph to visualise the reaction rates.

### The effects of pH on the rate of reaction

Enzymes require a specific pH to work best. Enzymes have a narrow range, and the optimum activity will be at the highest rate. This practical will investigate the optimum pH for the amylase enzyme.

Amylase is a catalyst for the hydrolysis of starch (amylose) to maltose. It is mostly present in plant tissue, which includes seeds. Starch will be broken down during seed germination to release glucose, a food source for the growing seed. Amylase works best at a neutral pH of 7 and a temperature of 37 ° C.

#### Material list

• Amylase enzyme solution
• Starch solution
• pH solution
• Iodine solution

#### Method

1. Set up the bunsen burner with the tripod over it - this is where you will place your beaker with the solution.
2. Place the beaker containing water on the tripod above the bunsen burner and keep the temperature at 35 degrees (adjust the flame accordingly).
3. Add a couple of drops of iodine solution onto the spotting tile.
4. In the test tube - mix 2 cm3 of amylase, 2 cm3 of starch and 1 cm 3 of pH solution.
5. Place the test tube in the beaker with water.
6. Every 20 seconds pipette a few drops of solution on the spotting tile (containing iodine solution).
7. Do this until the iodine solution no longer turns black, and note the time.
8. You can repeat this with different pH solutions.

Changing different independent variables, such as the pH or concentration of the enzyme or substrate, will allow you to investigate their effects on reaction rate.

Another enzyme you could potentially use is pepsin. Pepsin is an enzyme that catalyses the breakdown of proteins. Pepsin is present in most animal stomachs, and unlike most enzymes, its optimum pH is very low. This means it thrives in acidic environments.

### The effect of changing substrate concentration on rate of reaction

During cellular respiration, your mitochondria produce hydrogen peroxide (H202) as a by-product of oxygen combustion (substances react with oxygen to give off heat). Hydrogen peroxide is toxic to cells, so how does the body get rid of it to avoid harm? Several enzymes, including catalase, break down hydrogen peroxide. When it is broken down, it produces oxygen and water.

In this practical, you will investigate how different concentrations of hydrogen peroxide affect oxygen production.

#### Material list

• Hydrogen peroxide at a range of concentrations (example concentrations can include: 1M, 0.8M, 0.6M, 0.4M, 0.2M)
• Potato which has been cubed (this will act as the source of catalase)
• Large bowl
• Measuring cylinder

#### Method

1. Weigh the potato pieces and place them in the conical flask filled with 5cm3 hydrogen peroxide.
2. Fill a large bowl with water.
3. Fill the measuring cylinder and place it upside down inside the large bowl (make sure that no air bubbles are present).
4. The bung in the lid of the conical flask will have a tube extending out, which will go under the measuring cylinder.
5. Record the volume of oxygen in the measuring cylinder at regular time intervals (e.g. every 10 seconds).
6. Repeat with other hydrogen peroxide concentrations.

Increasing the concentration of hydrogen peroxide, i.e., the substrate will increase the reaction rate. However, this will not go on forever (unless an unlimited amount of enzyme is available), so when all enzyme active sites are occupied, no more substrate can be converted.

### The effect of changing enzyme concentration on the initial rate of reaction

Another factor affecting the reaction rate is enzyme concentration. The more enzymes you have, the quicker the reaction rate until all active sites are occupied. In this practical, you will measure the absorbance using a colourimeter (measures light transmittance).

#### Material list

• Milk powder solution

• Trypsin solution (1%)

• Distilled water

#### Method

1. Dilute the stock trypsin solution to produce concentrations of 0.2, 0.4, 0.6 and 0.8 %.

2. In the control solution, add 2cm3 of stock trypsin (1%) and 2cm3 of distilled water. This solution will be used to set the absorbance to zero.

3. In your test solution, add 2cm3 of milk suspension and 2cm3 of stock trypsin (1%). Mix and place in the colourimeter to read the absorbance.

4. At regular 15 second intervals, record the absorbance reading. Do this for 5 minutes.

5. Now repeat this for the other trypsin concentrations.

You can use the rate of reaction formula in the previous section to calculate the reaction rate.

The initial reaction rate will be directly proportional to the concentration of the enzyme. If unlimited substrate was present as the enzyme concentration increases, this increase could continue forever!

When you plot the enzyme concentration against the reaction rate with your result, the points may not totally fall in a straight line. However, in the perfect world, this is how it will look (Figure 3).

Figure 3. The initial rate of reaction with increasing enzyme concentration. Source: Odeta Razmaite - StudySmarter Originals.

When there is a limitation in enzyme concentration, the reaction will initially increase until all enzymes are occupied. It will reach the plateau (a flat line at a specific reaction rate).

## Enzyme Controlled Reactions - Key takeaways

• Enzymes are biological catalysts that increase the rate of reaction.
• The reaction rate is affected by factors including pH, temperature, enzyme and substrate concentrations.
• You can measure how each factor affects the reaction rate by setting up experiments and changing the independent variable (i.e. temperature, pH etc.).
• Many different enzymes speed up chemical reactions, including trypsin, catalase, and amylase. Some enzymes are involved in digestion, waste break down and other metabolic processes.

An increase in substrate concentration will increase the rate of reaction, then it will plateau. The plateau occurs when all the enzyme active sites are occupied.

The optimum pH will be different for each enzyme. Any variation in the optimum pH will reduce the rate of reaction and can also denature enzymes. This is because the amount of H+ ions will affect the tertiary structure, and therefore the active site of the enzyme.

Enzymes can be tested using different experiments.

For example, the effect of pH on enzymatic rate can be tested by placing enzymes in different concentrations of acid/alkaline solutions.

Enzyme controlled reactions can be controlled by factors such as:

• Temperature
• pH
• Substrate concentration
• Enzyme concentration
• Presence of inhibitors
• Presence of enzyme activators

Enzyme activity can be affected by factors such as temperature, pH, and enzyme concentration.

Two main kinds: anabolic and catabolic.

## Final Measuring enzyme-controlled reactions Quiz

Question

What methods can be used to track the progress of enzyme-catalyzed reactions:

The following methods can be used to track the progress of enzyme-catalyzed reactions:

1. Determining the rate at which a product is formed

2. calculating the rate at which a substrate vanishes

Show question

Question

What is amaylse?

Amylase  is a digestive enzyme that  hydrolyses starch into maltose and glucose.

Show question

Question

What is the pH of amylase

Amylase works best at a pH of 7

Show question

Question

What are the factors effecting enzyme reactions?

The factors effecting enzyme reactions are

• Level of crowding

• Salt concentration

• Effects of Temperature

• Effects of pH

• Substrate saturation

Show question

Question

Why enzymes are important to monitor?

Monitoring the rise or fall of enzyme levels can aid in the diagnosis of a variety of conditions.

Show question

Question

What is enzyme unit?

An enzyme unit is the amount of enzyme required to catalyze the transformation of 1 μmol of substrate per minute under specified pH and temperature conditions.

Show question

Question

What are the biological catalysts in body?

enzymes

Show question

Question

What is Catalyse?

Catalyse is a wide spread enzyme found in all animals, plants and it protects the cell from toxic effects of hydrogen peroxide. Catalase decomposes, or breaks down, hydrogen peroxide into water and oxygen.

Show question

Question

What is studies while investigating amylase activity using iodine

The rate of substrate disappearance is used to compare rates of reaction under different conditions

Show question

Question

How is enzyme activity measured?

Enzyme activity is measured in micromoles of substrate converted (or product created) per minute

Show question

Question

What components are formed when hydrogen peroxide breaks?

Catalase decomposes, or breaks down, hydrogen peroxide into water and oxygen.

Show question

Question

What is the colour of iodine solution without starch?

The iodine solution remains yellow-brown if no starch is present.

Show question

Question

What graph is plotted in investigations with Catalytic

A graph comparing the rate of oxygen production vs hydrogen peroxide concentration

Show question

Question

What is H2O2

Hydrogen Peroxide

Show question

Question

What chemical is used in the investigations with catalytic

Hydrogen peroxide solution

Show question

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