Question: Predict the effect of deleting the leader peptide sequence on regulation of the trp operon.
The deletion of the leader peptide sequence on the regulation of the trp operon would lead to the increment of the expression of the structural gene of the trp operon by attenuation.
Leader peptide sequence is present in trp operon that plays an important role in transcription.
Attenuation is a regulatory or terminatory mechanism that ensures proper transcription and translation.
The trp operon expresses ON (if low) and OFF(if high) mechanisms with respect to the levels of tryptophan in the cell. When the level of tryptophan is high, attenuation would cause premature termination while RNA polymerase is transcribing the trp operon.
Question: Even relatively compact bacterial genomes include some noncoding DNA between genes; large eukaryotic genomes are mostly noncoding DNA. How have genome comparisons helped identify noncoding sequences that have some biological function? Have the functions of any of these sequences been identified? Where are such sequences located?
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